Isolation and characterization of GD3 ganglioside having a novel disialosyl residue from rabbit thymus.
نویسندگان
چکیده
A major component of the di- and trisialoganglioside fraction of rabbit thymus was isolated. By neuraminidase treatment, methylation analysis, and CrO3 oxidation, it was identified as GD3 ganglioside with the novel disialosyl residue, NeuNGlyc(alpha, 2-8)NeuNAc(alpha, 2-3)Gal-(beta, 1-4)Glc(beta, 1-1)ceramide. Its concentration was 21.92 nmol/g of wet tissue (12.10% of the total gangliosides) and it contained 91.52% of the lipid-bound sialic acid in di- and trisialogangliosides of rabbit thymus.
منابع مشابه
Expression of GD3 ganglioside in childhood T-cell lymphoblastic malignancies.
Lymphoblasts from seven children with T-cell lymphoblastic malignancies and three children with non-T, non-B acute lymphoblastic leukemia (ALL) were analyzed for ganglioside content. Nonmalignant T-cells from thymus served as controls. Both ganglioside and glycoprotein sialic acid were increased approximately 3-3.5-fold in T-cell disease compared to thymic tissue when expressed on a per cell ba...
متن کاملNatural occurrence of ganglioside lactones. Isolation and characterization of GD1b inner ester from adult human brain.
A new ganglioside containing an inner ester linkage was extracted from adult brain specimens, obtained at the time of surgery on 51-70-year-old subjects, purified, and analyzed. It contains glucose, galactose, N-acetylgalactosamine, an N-acetylneuraminic acid in the molar ratio 1:2:1:2, but, on ion-exchange chromatography, behaves as a monosialoganglioside. Structural analyses showed its basic ...
متن کاملInduction of IgG antibodies against GD3 ganglioside in rabbits by an anti-idiotypic monoclonal antibody.
Anti-idiotypic MAb were raised in syngeneic mice against a mouse MAb recognizing GD3 ganglioside (MAb R24). Two anti-idiotypic MAb, designated BEC2 and BEC3, recognized distinct determinants on MAb R24 that mapped near or within the GD3-binding site. New Zealand white rabbits, which express GD3 on normal tissues, were immunized with either BEC2, BEC3, or control MAb FLOPC-21. All rabbits develo...
متن کاملExpression of GD3 Ganglioside in Childhood T-Cell Lymphoblastic Malignancies1
Lymphoblasts from seven children with T-cell lymphoblastic malig nancies and three children with non-T, non-B acute lymphoblastic leu kemia (ALL) were analyzed for ganglioside content. Nonmalignant I cells from thymus served as controls. Both ganglioside and glycoprotein sialic acid were increased approximately 3-3.5-fold in T-cell disease compared to thymic tissue when expressed on a per cell ...
متن کاملIsolation and Characterization of the Progenitor Cells From the Blastema Tissue Formed at Experimentally-Created Rabbit Ear Hole
Objective(s): Throughout evolution, mammalians have increasingly lost their ability to regenerate structures however rabbits are exceptional since they develop a blastema in their ear wound for regeneration purposes. Blastema consists of a group of undifferentiated cells capable of dividing and differentiating into the ear tissue. The objective of the present study is to isolate, culture expa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 253 22 شماره
صفحات -
تاریخ انتشار 1978